L-isoleucine is the L-enantiomer of isoleucine. It has a role as a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a plant metabolite, a human metabolite, an algal metabolite and a mouse metabolite. It is an aspartate family amino acid, a proteinogenic amino acid, an isoleucine and a L-alpha-amino acid. Experimental spectrum in Natural Products Magnetic Resonance Database: [NP0000645](https://np-mrd.org/natural_products/485/submission_spectrum_view?spectrum_id=300&spectrum_type=real)