Frozen frog - muscle

Description:
Muscle from frozen and control moor frog Rana arvalis. *Reference*: S.V. Shekhovtsov, N.A. Bulakhova, Yu.P. Tsentalovich, E.A. Zelentsova, E.N. Meshcheryakova, T.V. Poluboyarova, D.I. Berman. Metabolomic analysis reveals that the moor frog Rana arvalis uses both glucose and glycerol as cryoprotectants. Animals 2022, 12, 1286. https://doi.org/10.3390/ani12101286
Property Value
Experiment name Frozen frog - muscle
Experiment ID 251
Sample groups Frog muscle control, Frog muscle frozen
Species Moor frog (Rana arvalis)
Tissues Muscle
Diseases N/A
Date of creation 14:30, May 10, 2023
Date of last modification 09:26, Sep 07, 2023
Author v.yanshole
Charts:
Concentration table
Average concentrations of metabolites in groups. Average ± Std.Dev., in nmol per gram of wet tissue (nmol/g).
Metabolites / Groups Frog muscle control Frog muscle frozen
1-Methylhistidine 133.3 ± 50.0 1388.3 ± 2643.0
2,3-Butanediol 8.1 ± 3.5 16128.3 ± 28660.7
2-Aminoadipic acid 420.4 ± 155.0 605.3 ± 388.4
2-Aminobutyric acid 22.5 ± 6.9 58.4 ± 65.4
2-Hydroxyisobutyric acid 3.2 ± 2.2 30.8 ± 26.0
3-Aminoisobutyric acid 324.9 ± 190.3 2109.0 ± 3478.2
4-Aminobutyric acid 27.8 ± 30.6 95.4 ± 89.3
Acetic acid 3698.6 ± 974.6 8856.9 ± 7624.3
Acetylcarnitine 28.6 ± 18.3 274.1 ± 298.4
Adenosylhomocysteine N/A 85.4 ± 79.5
ADP 595.6 ± 201.1 1524.2 ± 1596.0
Alanine 4628.4 ± 2534.7 24075.6 ± 24667.9
AMP 26.5 ± 8.0 632.9 ± 385.5
Asparagine 627.6 ± 363.0 962.3 ± 1273.0
Aspartic acid 1243.8 ± 471.8 249.5 ± 276.2
ATP 1505.7 ± 531.3 1135.8 ± 1319.3
Carnosine 11566.3 ± 4715.6 27826.1 ± 20745.5
Choline 40.2 ± 16.3 738.8 ± 1062.3
Creatine 15815.4 ± 4693.3 79808.0 ± 55768.1
Dimethylglycine 1190.1 ± 304.1 N/A
Dimethylmalonic acid 127.8 ± 40.9 1181.9 ± 1154.0
Ergothioneine 46.4 ± 20.5 202.3 ± 188.5
Ethanol 35.8 ± 25.9 76.0 ± 32.3
Formic acid 172.5 ± 53.7 377.8 ± 297.2
Fumaric acid 50.0 ± 55.4 40.6 ± 39.5
Glucose 1610.0 ± 1380.3 80536.5 ± 140755.3
Glutamic acid 5362.7 ± 3256.6 3120.5 ± 4619.0
Glutamine 391.8 ± 158.5 1066.1 ± 1274.1
Glycerol 767.7 ± 662.7 239472.2 ± 207040.4
Glycerophosphocholine 3641.1 ± 1678.8 14274.8 ± 12999.5
GSH 320.2 ± 114.1 503.6 ± 436.2
GTP 77.9 ± 26.6 58.3 ± 61.9
Hypoxanthine 10.3 ± 4.6 885.4 ± 1609.2
IMP 20.2 ± 12.7 1629.9 ± 2347.5
Isoleucine 51.7 ± 28.7 508.4 ± 986.1
Ketoleucine 114.9 ± 56.2 1761.3 ± 2998.3
L-Lactic acid 1559.9 ± 1187.5 53670.1 ± 63592.4
Malic acid 437.6 ± 186.4 1099.5 ± 733.0
Maltitol N/A 32.2 ± 20.1
Maltose 347.0 ± 134.7 806.8 ± 1047.9
Mannose 174.5 ± 182.0 2980.7 ± 5528.7
Methionine 199.7 ± 110.9 410.3 ± 792.9
Methionine sulfoxide 7.5 ± 2.5 33.7 ± 35.7
N-acetyltyrosine 30.7 ± 19.7 434.9 ± 767.3
NAD 288.1 ± 95.7 404.9 ± 264.6
NADH 13.6 ± 4.8 67.5 ± 51.6
NADPH 47.7 ± 31.9 421.2 ± 369.6
Pantothenic acid 6.5 ± 3.4 49.3 ± 71.9
Phenylalanine 34.5 ± 20.3 699.5 ± 1227.7
Phosphocreatine 10795.8 ± 5136.4 1974.7 ± 1377.5
Pyruvic acid 29.7 ± 36.3 48.2 ± 63.7
Ser-PETA 4164.8 ± 1930.7 7634.7 ± 7129.6
Succinic acid 70.1 ± 59.2 1911.5 ± 1275.0
Threonine 732.3 ± 298.3 1972.6 ± 1421.1
Uracil N/A 219.9 ± 300.3
Valine 96.3 ± 43.3 821.5 ± 1564.2
Full concentration table
Concentration of metabolites in samples, in nmol per gram of wet tissue (nmol/g).
Metabolites / Samples MRN6 MRN9 MRN11 MRN12 MRN13 MRF8 MRF10 MRF12 MRF13 MRF14
1-Methylhistidine 177.0 169.9 53.6 119.0 147.2 334.3 50.2 416.2 34.4 6106.5
2,3-Butanediol 11.7 9.1 7.2 9.9 2.6 59108.9 1962.9 2481.8 959.6 N/A
2-Aminoadipic acid 265.4 646.9 467.9 285.1 436.7 890.5 92.5 708.0 320.9 1014.7
2-Aminobutyric acid 29.4 26.8 17.2 13.4 25.8 147.2 2.8 27.3 6.5 108.1
2-Hydroxyisobutyric acid 2.4 5.5 0.8 1.7 5.5 36.9 6.4 24.1 14.0 72.6
3-Aminoisobutyric acid 151.2 568.3 115.9 362.9 426.3 1219.4 69.4 848.1 137.8 8270.5
4-Aminobutyric acid 10.3 12.2 81.8 22.6 12.0 236.7 19.3 70.0 27.0 124.0
Acetic acid 5140.3 3493.2 2403.7 3727.1 3728.7 19833.2 1940.2 5348 3495.5 13667.5
Acetylcarnitine 54.5 26.5 8.4 38.3 15.6 794.2 74.9 116.5 130.4 254.6
Adenosylhomocysteine N/A N/A N/A N/A N/A 196.3 12.7 57.1 22.2 138.9
ADP 865.1 650.5 334.7 474.2 653.3 4119.7 840.3 1923.1 647.7 90.0
Alanine 8891.0 3443.5 2952.8 5008.0 2847.0 65845.8 5894.6 24580.2 6188.5 17869.0
AMP 17.3 20.7 27.2 37.9 29.6 1052.4 308.3 1000.7 589.6 213.5
Asparagine 1132.9 649.0 193.5 779.4 383.3 2753.9 46.6 78.6 57.1 1875.5
Aspartic acid 1693.5 1152.2 486.7 1559.3 1327.2 447.9 26.0 46.6 90.9 636.2
ATP 1740.3 2224.8 808.9 1515.8 1238.8 3423.2 578.9 1060.5 456.1 160.0
Carnosine 16595.4 14781 5667.1 7585.9 13202.2 40091.0 5628.7 25407.8 11683.0 56320.1
Choline 57.5 50.9 19.3 26.8 46.5 936.7 29.2 116.3 93.3 2518.4
Creatine 21155.3 16908.5 8235.0 16017 16761.4 138807.9 20414.3 67247.2 35344.0 137226.8
Dimethylglycine 1434 1237.1 692.7 1152.6 1433.9 N/A N/A N/A N/A N/A
Dimethylmalonic acid 158.5 133.9 66.3 168.5 111.7 2718.4 213.8 585.6 281.2 2110.4
Ergothioneine 79.4 39.5 45.8 44.0 23.2 371.7 28.6 66.2 106.9 438.2
Ethanol 10.8 45.3 11.9 37.9 73.0 91.2 20.9 82.7 105.2 80.2
Formic acid 239.3 138.3 101.6 183.3 200.2 632.2 112.7 210.2 171.7 762.0
Fumaric acid 145.0 14.4 7.4 38.2 44.9 92.3 4.9 13.6 18.6 73.3
Glucose 4056.8 1306.6 813.2 955.4 918.1 331666.5 29460.9 2025.5 16388.0 23141.7
Glutamic acid 8654.2 8576.7 1016.3 4418.7 4147.7 2054.5 253.3 1352.3 651.2 11291.1
Glutamine 508.0 552.5 150.2 340.9 407.4 2277.9 41.8 210.5 171.3 2629.2
Glycerol 1871.9 609.2 262.6 830.4 264.2 534701.6 77944.3 303849.8 271674.9 9190.3
Glycerophosphocholine 5759.8 3161.7 1740.9 4977.9 2565.3 31198.1 3067.3 7693.1 4213.8 25201.9
GSH 389.7 445.0 168.4 361.0 236.6 661.7 105.0 289.7 268.0 1193.5
GTP 94.8 109.4 47.6 53.4 84.3 167.6 24.7 39.9 17.7 41.6
Hypoxanthine 14.9 13.8 11.7 7.0 4.1 343.6 62.0 212.4 52.7 3756.1
IMP 21.6 4.1 39.0 15.0 21.4 767.4 308.6 563.2 692.7 5817.7
Isoleucine 50.5 74.7 14.3 34.4 84.3 180.4 6.6 74.5 12.6 2267.9
Ketoleucine 126.2 143.1 33.4 90.3 181.6 1481.5 45.8 188.6 74.3 7016.6
L-Lactic acid 3615.7 702.4 803.5 1408.5 1269.3 54467.1 5563.9 31324.8 14551.7 162443.1
Malic acid 487.7 628.0 128.1 440.5 504.0 1993.4 278.8 1217.4 436.5 1571.6
Maltitol N/A N/A N/A N/A N/A 44.8 14.9 15.3 53.8 N/A
Maltose 494.7 328.3 350.3 425.2 136.6 2501.2 168.5 50.4 158.6 1155.6
Mannose 497.9 88.8 71.0 85.6 129.3 12800.3 318.9 35.3 97.2 1651.9
Methionine 334.6 272.2 46.9 193.5 151.4 129.4 7.6 68.4 20.1 1826.2
Methionine sulfoxide 10.8 7.4 3.7 7.7 7.9 88.5 1.7 33.7 2.4 42.2
N-acetyltyrosine 46.6 49.1 0.6 23.7 33.3 248.9 16.2 98.8 13.8 1796.9
NAD 359.8 359.8 126.3 295.2 299.5 821.7 191.4 487.6 342.5 181.2
NADH 11.2 20.8 9.0 16.1 11.1 156.3 22.4 61.1 47.3 50.1
NADPH 99.2 56.9 21.0 35.3 26.1 896.5 56.2 247.9 174.1 731.2
Pantothenic acid 11.7 7.8 4.4 3.1 5.5 50.6 5.1 9.6 7.5 173.5
Phenylalanine 43.9 49.7 2.5 26.4 50.2 157.3 17.4 84.2 N/A 2539
Phosphocreatine 15374.9 16809.2 4582.5 8284.6 8927.9 4085.8 270.5 1947.6 2064.6 1504.9
Pyruvic acid 94.3 18.8 7.8 15.6 12.1 157.0 6.7 11.3 12.7 53.5
Ser-PETA 4259.8 7336.3 2195.6 3764.7 3267.4 19840.0 2266.6 6941.0 2804.2 6321.6
Succinic acid 37.2 30.4 18.1 152.9 112.2 3509.2 340.7 1255.7 1572.2 2879.7
Threonine 1236.6 684.7 517.8 501.7 720.5 3144.9 456.8 2290.1 518.5 3452.8
Uracil N/A N/A N/A N/A N/A 286.4 24.0 51.4 18.8 719.0
Valine 100.9 121.5 36.4 74.1 148.7 339.4 18.3 114.4 25.4 3610.0
Metadata
Properties / Samples MRN6 MRN9 MRN11 MRN12 MRN13 MRF8 MRF10 MRF12 MRF13 MRF14
Group Frog muscle contr… Frog muscle contr… Frog muscle contr… Frog muscle contr… Frog muscle contr… Frog muscle frozen Frog muscle frozen Frog muscle frozen Frog muscle frozen Frog muscle frozen
Description N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Species Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a… Moor frog (Rana a…
Tissue Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle Muscle
Diseases N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Gender N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Origin wild wild wild wild wild wild wild wild wild wild
Sample weight, mg 86.3 128.1 176.5 112.6 114.3 35.5 140.0 56.8 120.2 38.5
Sampling place Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia Chernogolovka, Russia
Sampling date Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020 Sept. 27, 2020
Time postmortem, min 5 5 5 5 5 5 5 5 5 5
Raw files
File types / Samples MRN6 MRN9 MRN11 MRN12 MRN13 MRF8 MRF10 MRF12 MRF13 MRF14
MS N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
NMR
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